Microbiome

Microbiome Analysis

16S ribosomal RNA (rRNA) gene sequencing is a common amplicon sequencing method used to identify and compare bacteria present within a given sample. Next-generation sequencing enables analysis of the entire microbial community within a sample. With the ability to combine many samples in a sequencing run, researchers can use NGS-based 16S rRNA gene sequencing as a cost-effective technique to identify strains that may not be found using other methods. In collaboration with the Duke Center for Genomics of Microbial Systems (GeMS), we are providing experimental design, full sample processing, and analysis consultation.

Service Pricing and Summary

Sample types will need to be collected in facility provided tubes:

  • Fecal swabs or 0.1 gram of solid fecal material
  • Watery stool sample of 750 ul
  • Soil samples of 0.3 grams
  • Swabs or other sample types

Microbiome 16S Service Details:

  • MoBio PowerSoil DNA Extraction
  • Library prep (pcr amp with barcoding)
  • Amplicon purification, quantification, and pooling
  • Illumina MiSeq Sequencing 150bp PE or 250bp PE

Note: Barcoding up to 192 pooled samples in one MiSeq lane

Sequencing Primers:

For information on the primers used for paired-end community sequencing on the Illumina HiSeq or MiSeq, please refer to this paper .