- Will you send me the QC results before you start my order?
- May I submit my samples for QC before I decide which ones I want to use?
- How long will it be before my samples are sequenced?
- May I have my samples back?
- What is the shipping address?
- How do I create an order?
- How do I submit my samples?
- I’m preparing a grant application; can anyone help me with the sequencing portion?
- How much does it cost?
- How would you like samples to be shipped?
- When do I get invoiced?
- What are the payment modes?
- How do I get my data?
- What happens to my samples afterwards?
- How long will my data be available?
- Do you run QC on my samples?
- What are the input requirements for your Illumina library preps?
- May I submit my own prepared libraries? What are the requirements?
- What if my library sample contains two different fragment sizes or is a very broad range of sizes? What does this mean?
- What results do you guarantee from investigator prepared libraries?
- What type of DNA extraction kits do you recommend for PacBio sequencing?
- How do I download my sequencing data?
- How do I share my data with collaborators?
- How do I acknowledge the sequencing core in publications?
How long will it be before my samples are sequenced?
Average queue times are posted on our home page. The length of time will depend on the type of run, whether we are preparing your libraries and our current volume of requests. It may also be influenced by kit availability and any unforeseen technical issues.
Be assured that we do our best to generate your data as soon as possible.
What is the shipping address?
The shipping address is
Att: [insert sequencing platform you have requested]
Sequencing and Genomic Technologies Shared Resource
701 W. Main St.
Durham, NC, 27701
I’m preparing a grant application; can anyone help me with the sequencing portion?
Yes, email firstname.lastname@example.org or schedule an consultation with us and we would be happy to discuss your project.
- Next generation sequencing: if you know which service you would like, you can use DUGSIM to estimate the cost of your project.
- Using the “Estimate Cost” button you can select the services that you require and submit the request for a quote. See our Pricing and Ordering page for more details.
- If you are unfamiliar with the different technologies, and desire a consultation, contact us.
- After you have received your order number, samples should be shipped on blue ice or dry ice.
- Please include a print out of your order with your shipment.
- Detailed information about how and where to ship your samples can be found here.
- Duke clients must supply a fund code before an order is generated.
- Non- Duke clients must provide a signed PO before an order number will be issued. Payments may be made by check or wire transfer.
- We do not accept credit cards.
How do I get my data?
For our Duke customers with a NetID, the data is uploaded to Duke Data Service. An email with information on how to accept and access the data will be sent to the researcher who ordered the sequencing.
For our non-Duke customers, data will be uploaded to an sftp server. Login and password to access the data will be sent by email once the data has been uploaded.
- Samples will be kept for three months after the sequencing data is released.
- After that, they will be discarded unless retention has been requested. You can either add a comment to that effect in the comment box of your quote/order or email us at email@example.com
- Yes, we make a Qubit measurement followed by a Bioanalyzer or Tapestation evaluation.
- If your samples look questionable or disagree with the information you supplied, we will contact you before proceeding.
What are the input requirements for your Illumina library preps?
Sample requirements can be found here.
May I submit my own prepared libraries? What are the requirements?
Yes, you may submit your own library(s). Requirements will vary depending on the sequencer you plan on using. Requirements can be found here.
Note that we do not guaranetee the sequencing results for any customer prepared libraries.
- It will be very difficult to accurately calculate the molarity of your sample.
- If we can’t accurately determine the correct molarity of your library, it can lead to under or overloading of the flow cell.
- You will be asked to decide which fragment size you want to choose for the calculation, or if you want to proceed.
- As is the case with any customer prepared libraries, we do not guaranetee the sequencing results.
What results do you guarantee from investigator prepared libraries?
If your sample appears to be sequenceable based on our QC data, we will proceed. However, we do not guarantee results with investigator prepared libraries.
- PacBio recommends Qiagen HMW gDNA extraction kits such as MagAttract.
- Please indicate your extraction protocol when you submit your sample. gDNA samples need an OD 260/280 ratio of approximatively 1.8 to 2.
- For PCR products, gel cuts may be done, but the gel can not be exposed to ethidium bromide and UV light. Use instead CYBR green and blue light. PCR products must be cleaned prior to submission using either AMPure XP beads or Qiagen PCR cleanup spin columns.
- More information about sample requirements
If your sequencing data was delivered using Duke Data Service, the best way to download it is to use the DDSClient tools.
View detailed information on how to use DDSclient tools
If your sequence data was delivered using sftp, you should have received an email with a login and password. You can download your data using unix command line (sftp) or a sftp client such as FileZilla.
If your data was delivered using sftp, you can simply forward the email containing the login and password to your collaborators.
For all publications that include data generated in the Sequencing and Genomic Technologies Shared Resource, we kindly request that you acknowledge this support:
We thank the Duke University School of Medicine for the use of the Sequencing and Genomic Technologies Shared Resource, which provided _________ service.