FAQs

Frequently Asked Questions


Will you send me the QC results before you start my order?
We only send QC results if there is a problem with your samples, or you have specifically requested them before we proceed with your order. 

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May I submit my samples for QC before I decide which ones I want to use?
Yes, but you MUST have a sequencing order, and MUST submit ample volume of sample for us to proceed with the order.

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How long will it be before my samples are sequenced?
Average queue times are posted on our home page. The length of time will depend on the type of run, whether we are preparing your libraries and our current volume of requests. It may also be influenced by kit availability and any unforeseen technical issues.

Be assured that we do our best to generate your data as soon as possible.

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May I have my samples back?
Yes, but please indicate in the comment box of your quote/order that you want your samples back.

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What is the shipping address?
The shipping address is
Att: [insert sequencing platform you have requested]
Duke University
Sequencing and Genomic Technologies Shared Resource
Chesterfield bldg.
701 W. Main St.
Suite 320
Durham, NC, 27701
USA

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How do I create an order?
All requests must be made using DUGSIM. Visit our pricing and ordering page for more information on how to use DUGSIM.

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How do I submit my samples?
No samples may be accepted until you have placed an order in DUGSIM. Visit our sample submission guidelines for more information.

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I’m preparing a grant application; can anyone help me with the sequencing portion?
Yes, email sequencing@duke.edu or schedule an consultation  with  us and we would be happy to discuss your project.

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How much does it cost?

  • Next generation sequencing: if you know which service you would like, you can use DUGSIM to estimate the cost of your project. 
  • Using the “Estimate Cost” button you can select the services that you require and submit the request for a quote.   See our Pricing and Ordering page for more details.
  • If you are unfamiliar with the different technologies, and desire a consultation, contact us.

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How would you like samples to be shipped?

  • After you have received your order number, samples should be shipped on blue ice or dry ice. 
  • Please include a print out of your order with your shipment.
  • Detailed information about how and where to ship your samples can be found here.

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When do I get invoiced?
Invoices are generated after the sequencing results have been released.

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What are the payment modes?

  • Duke clients must supply a fund code before an order is generated. 
  • Non- Duke clients must provide a signed PO before an order number will be issued. Payments may be made by check or wire transfer.
  • We do not accept credit cards.

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How do I get my data?
Data is uploaded to an sftp server and availabe to download. Instructions for how to download your data will be sent to you once your data has been released.

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What happens to my samples afterwards?

  • Samples will be kept for two months after the sequencing data is released. 
  • After that, they will be discarded unless retention has been requested on the original submission form.

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How long will my data be available?
Unfortunately, we do not have enough storage capacity to keep your data indefinitely on our server. It will thus be removed after 8 weeks. 

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Do you run QC on my samples?

  • Yes, we make a Qubit measurement followed by a Bioanalyzer or Tapestation evaluation. 
  • If your samples look questionable or disagree with the information you supplied, we will contact you before proceeding.

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What are the input requirements for your Illumina library preps?
Sample requirements can be found here.

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May I submit my own prepared libraries? What are the requirements?
Yes, you may submit your own library(s). The concentration of your library should be >10nM in more than 15μl.

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What if my library sample contains two different fragment sizes or is a very broad range of sizes? What does this mean?

  • It will be very difficult to accurately calculate the molarity of your sample. 
  • If we can’t accurately determine  the correct molarity of your library, it can lead to  under or overloading of the flow cell.
  • You will be asked to decide which fragment size you want to choose for the calculation, or if you want to proceed. 
  • We are unable to guarantee your sequencing results if the QC of your library does not meet our expectations.

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What results do you guarantee from investigator prepared libraries?
If your sample appears to be sequenceable based on our QC data, we will proceed. However, we do not guarantee results with investigator prepared libraries.  

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What type of DNA extraction kits do you recommend for PacBio sequencing?

  • PacBio recommends Qiagen HMW gDNA extraction kits such as MagAttract.
  • Please indicate your extraction protocol when you submit your sample. gDNA samples need an OD 260/280 ratio of approximatively 1.8 to 2.
  • For PCR products, gel cuts may be done, but the gel can not be exposed to ethidium bromide and UV light. Use instead CYBR green and blue light. PCR products must be cleaned prior to submission using either AMPure XP beads or Qiagen PCR cleanup spin columns. 
  • More information about sample requirements

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