DNA Quality Check

DNA Quality Check

Our DNA quality check service includes the following options:

  • Quality check with Agilent Bioanalyzer to assess DNA fragment sizes and concentrations.
  • Quality check with Qubit to assess sample concentration.

Contact microarraylab@duke.edu or call (919)668-1583 for pricing.

Investigators that wish to use DNA Quality Check service should use our online submission forms to initiate the process.

Agilent Bioanalyzer

person using the agilent bioanalyzer

The Agilent Bioanalyzer DNA assay leverages microfluidics technology, enabling the quality analysis of DNA using only 1ul of sample. The assay is run on the Agilent 2100 Bioanalyzer instrument and utilizes the Agilent 2100 Expert Software to analyze and display results. DNA fragement sizes and concentrations is generated for each sample.

Qubit Fluorometer

qubit fluorometer

The Qubit 2.0 Fluorometer quantitates DNA, RNA, and protein with unprecedented accuracy, sensitivity, and simplicity. It is designed for molecular biology labs that work with precious samples that are rare or difficult to process, and applications requiring precise measurement such as real-time PCR.

Assay TypeSize RangeConcentration Rangemax # of samples per chiprecommended DNA types
DNA 100025-1000 bp0.5-50 ng/ul12PCR products, restriction digest or fragmented DNA
High Sensitivity DNA50-7000 bp0.005-0.5 ng/ul11

fragmented DNA or DNA libraries in pg/ul range

Qubit dsDNA BRany0.1-1000 ng/ulNAgenomic DNA