Sequencing and Genomic Technologies

Sequencing and Genomic Technologies

scientist in the lab

Next-Generation Sequencing Solutions
Illumina, PacBio, NGS Library preparation including single-cell RNA-seq

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The Sequencing and Genomic Technologies Shared Resource is a basic research oriented core facility affiliated with the Duke Cancer Institute (DCI). This Shared Resource has a track record more than a decade-long of providing constantly updated, state-of-the-art genomic services. The Sequencing and Genomic Technologies Shared Resource offers the full range of genomic technologies, making it much simpler for researchers to find the right service for their needs.

Director Nicolas Devos is available for face-to-face project consultations on campus in the CIEMAS building or at our new facility. 

Schedule a project consulation

Service update

11/22/19 Thanksgiving closure:
The facility in CIEMAS and Chesterfield will be unavailable for sample submission Wednesday November 27, Thursday November 28 and Friday November 29. We will reopen on December 1. Have a safe holiday!

Nucleic Acid Extraction Services

We now provide Nucleic Acid extraction services on our QIAsymphony robot. The new extraction services provide researchers with fast turnaround time, high reproducibility through standardized processing of large sample batches (96 samples at a time) and seamless integration with our NGS library preparation services.

We currently offer total RNA extraction from blood samples in PAXgene tubes, total RNA extractions from cell pellets and miRNA extraction from serum/plasma.  Please visit our sample requirement page for more information on how to submit your samples.

Don’t hesitate to contact us if you have large amount of samples to extract.  The QIAsymphony can automate many types of DNA and RNA extractions from a broad range of sample types.

Average Time in Queue

Client-Submitted LibrariesCore-Created Libraries
MiSeq2 weeks3-4 weeks
NextSeq10 days3-4 weeks
NovaSeq 6000 full flow cells10 days3-4 weeks
Novaseq 6000 by the laneDependent on how quickly a flow cell can be filledDependent on how quickly a flow cell can be filled
PacBioSequelNot applicable2 weeks

Announcements

May 2, 2019

Due to the low demand and increasing costs for sequencing on our Illumina HiSeq 4000, we have made the decision to retire it, effective July 1, 2019.