Next-Generation Sequencing Solutions
Illumina, PacBio, NGS Library preparation including single-cell RNA-seq
The Sequencing and Genomic Technologies Shared Resource is a basic research oriented core facility affiliated with the Duke Cancer Institute (DCI). This Shared Resource has a track record more than a decade-long of providing constantly updated, state-of-the-art genomic services. The Sequencing and Genomic Technologies Shared Resource offers the full range of genomic technologies, making it much simpler for researchers to find the right service for their needs.
Director Nicolas Devos is available for face-to-face project consultations on campus in the CIEMAS building or at our new facility.
11/22/19 Thanksgiving closure:
The facility in CIEMAS and Chesterfield will be unavailable for sample submission Wednesday November 27, Thursday November 28 and Friday November 29. We will reopen on December 1. Have a safe holiday!
Nucleic Acid Extraction Services
We now provide Nucleic Acid extraction services on our QIAsymphony robot. The new extraction services provide researchers with fast turnaround time, high reproducibility through standardized processing of large sample batches (96 samples at a time) and seamless integration with our NGS library preparation services.
We currently offer total RNA extraction from blood samples in PAXgene tubes, total RNA extractions from cell pellets and miRNA extraction from serum/plasma. Please visit our sample requirement page for more information on how to submit your samples.
Don’t hesitate to contact us if you have large amount of samples to extract. The QIAsymphony can automate many types of DNA and RNA extractions from a broad range of sample types.
Average Time in Queue
|Client-Submitted Libraries||Core-Created Libraries|
|MiSeq||2 weeks||3-4 weeks|
|NextSeq||10 days||3-4 weeks|
|NovaSeq 6000 full flow cells||10 days||3-4 weeks|
|Novaseq 6000 by the lane||Dependent on how quickly a flow cell can be filled||Dependent on how quickly a flow cell can be filled|
|PacBioSequel||Not applicable||2 weeks|