Sequencing and Genomic Technologies

Sequencing and Genomic Technologies

scientist in the lab

Next-Generation Sequencing Solutions
Illumina, PacBio, NGS Library preparation including single-cell RNA-seq

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The Sequencing and Genomic Technologies Shared Resource is a basic research oriented core facility affiliated with the Duke Cancer Institute (DCI). This Shared Resource has a track record more than a decade-long of providing constantly updated, state-of-the-art genomic services. The Sequencing and Genomic Technologies Shared Resource offers the full range of genomic technologies, making it much simpler for researchers to find the right service for their needs.

Director Nicolas Devos is available for face-to-face project consultations on campus in the CIEMAS building or at our new facility. 

Schedule a project consulation

Nucleic Acid Extraction Services

We now provide Nucleic Acid extraction services on our QIAsymphony robot. The new extraction services provide researchers with fast turnaround time, high reproducibility through standardized processing of large sample batches (96 samples at a time) and seamless integration with our NGS library preparation services.

We currently offer total RNA extraction from blood samples in PAXgene tubes for $30 per sample.  Please visit our sample requirement page for more information on how to submit your samples.

Don’t hesitate to contact us if you have large amount of samples to extract.  The QIAsymphony can automate many types of DNA and RNA extractions from a broad range of sample types.

Average Time in Queue

Client-Submitted LibrariesCore-Created Libraries
Mi-Seq1 week3-4 weeks
NextSeq1 week3-4 weeks
HiSeq 2500 Rapid Run1 week3-4 weeks
HiSeq 4000 full flow cell1 week3-4 weeks
HiSeq 4000 by the lane3-4 weeks3-4 weeks
NovaSeq 6000 full flow cells1 week3-4 weeks
Novaseq 6000 by the laneDependent on how quickly a flow cell can be filledDependent on how quickly a flow cell can be filled
PacBioSequelNot applicable2 weeks

Announcements

February 28, 2018
The Sequencing Core has acquired a brand new QIAsymphony to perform high throughput nucleic acid extractions. We currrently have a service for RNA extractions from blood collected in PAXgene tubes. The QIAsymphony can automate many types of DNA and RNA extractions from a broad range of sample types. Contact us to discuss potential other nucleic acid extraction services.