Past GCB Academy Offerings

PAST Course Offerings

(return to current offerings)

Microbiome Analysis
Instructors: Holly Dressman & Olaf Mueller
Cost: Free
An introductory discussion on what is involved in designing and analyzing the microbiome.  We will cover study design, sample collection/storage/preparation/sequencing of 16S rDNA and provide a 3-4 hour analysis using basic QIIME data analysis.

Introduction to Mass Spec Technologies for Proteomics and Metabolomics
Instructor: Dr. Arthur Moseley, Director, Duke Proteomics and Metabolomics Shared Resource
Cost: Free

Liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) continues to be the key technology for the qualitative and quantitative analysis of peptides, proteins and metabolites for both basic and clinical research projects. This GCB Academy session is designed as an introduction for researchers needing to expand their knowledge of the use of LC/MS/MS-based methods for proteomics and metabolomics, and thus help researchers better understand how these technologies can help inform their research goals. Background material in basic protein/metabolite chemistries will be provided, with an emphasis on how to use the physicochemical characteristics of these biomolecules for sample preparation specifically for LC/MS/MS analyses. In addition, the fundamentals of liquid chromatography and mass spectrometry will be discussed to enable students to understand the nuances of the experimental designs required to address their specific project. Real-world examples will be used to illustrate sample preparation and analysis strategies, including basic identification projects, characterization of Post-Translational Modifications and differential expression analyses (including 'omic biomarker discovery and targeted biomarker verification). Finally, the use of open source software tools for interpretation of these datasets will be discussed.

Mass Spectrometry Based Proteomic and Metabolomic Data Analysis Strategies
Instructor: Dr. Erik Soderblom and Dr. Will Thompson, Duke Proteomics and Metabolomics 
Cost: Free

Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) is a versatile tool for the qualitative and quantitative characterization of peptides, proteins and metabolites for both basic and clinical research projects. One of the most important considerations in being able to translate LC-MS datasets into meaningful biological observations is to effectively use open source software packages and other online resources to perform proper data analysis, interpretation, and forward-looking experimental design.  This GCB Academy session is designed as a complement to “Introduction to Mass Spec Technologies for Proteomics and Metabolomics” and is intended for users of the Proteomics and Metabolomics Shared Resource who have or plan on generating LC/MS based Proteomic or Metabolomic Datasets with the Shared Resource. The first section of the course will focus on the effective use of Scaffold to characterize qualitative proteomic datasets. This will include an overview of Scaffold and features such as interpretation of spectral matches at a protein or peptide level, gene ontology classification, homology matching, spectral count data, and data export. The second section will cover the interpretation and meta-analysis of data provided from the quantitative proteomics and metabolomics pipelines (typically .xlsx file formats from Rosetta Elucidator) provided as part of the Shared Resource’s quantitative workflows. This will include an overview of the typical features of a quantitative data return document, various data summarization levels (e.g. peptide versus protein), calculating peptide/protein relative fold-changes and p-values, exporting data for motif analysis (PTM specific datasets), and performing Principle Component Analysis (PCA) and 2D Clustering within JMP Pro (SAS Institute, Cary, NC). Finally, we will cover the use of Skyline as a tool for targeted quantitative proteomics workflows.  This will include utilizing Skyline for verification following LC-MS based discovery experiments, as well as a brief introduction to using Skyline to design and interpret targeted proteomics and metabolomics analysis.   This portion will utilize hands-on analysis of raw data collected in the Shared Resource.  For advance training in Skyline, the tutorials on the Skyline software web site are highly recommended (https://skyline.gs.washington.edu/labkey/wiki/home/software/Skyline/page.view?name=tutorials).
Prerequisites:
1)         GCB Academy course; “Introduction to Mass Spec Technologies for Proteomics and Metabolomics” (optional, but preferred)
2)         Personal laptop with Scaffold (http://www.proteomesoftware.com/products/scaffold/download/), Skyline (https://skyline.gs.washington.edu/labkey/project/home/software/Skyline/begin.view?), and JMP Pro (downloaded from Duke OIT, https://software.oit.duke.edu/comp-print/software/index.php) pre-installed.

Single Cell Expression Profiling
Instructors: Dr. Holly Dressman, Co-Director, Sequencing and Genomic Technologies
Cost:  FREE

Single cell expression profiling can be facilitated through the automation of the Fluidigm C1 System.  The system allows one to capture single cells and explore gene expression profiling through the use of qPCR or RNA sequencing analysis.   The technology will be discussed as well as how it can be applied in your research.  Pre-requisites: Basic understanding of molecular biology.

Overview of PCR Technologies
Instructors: Dr. Holly Dressman, Co-Director, Sequencing and Genomic Technologies
Cost: FREE

PCR, quantitative PCR and droplet-digital PCR technologies will be discussed along with examples on which technology would best fit your research.

RNA-Seq Library Construction (limit 12 participants)
2.5 days
Instructors: Dr. Olivier Fédrigo, Director, and Dr. Nicolas Devos, Associate Director,  Sequencing and Genomic Technologies Shared Resource
Cost: $200

In this 3-day workshop, participants will prepare stranded RNA-Seq libraries and will have the opportunit to generate and analyze expression data. This hands-on workshop consists of two parts: 1) sample preparation and data generation (wet lab) and 2) data analysis. In the first part, participants will be trained at estimating RNA sample quality, generating stranded directional RNA-Seq libraries, and assessing RNA-Seq library quality. In the second part, participants will learn how to perform basic bioinformatics analyses on the RNA-Seq data, including data QC, mapping reads, and differential expression analysis. For more in-depth analyses, the GCB Academy course on RNA-Seq analysis is recommended.

Pre-requisites: Attendees should have basic laboratory skills such as lab safety principles, best RNA practices, pipetting, and dilutions.

Variant Discovery
Instructor: Dr. David Corcoran, Director, Genomic Analysis and Bioinformatics Shared Resource
Cost: $50 for faculty, postdocs, and staff; free for graduate students

This hands-on tutorial will introduce the data processing steps for the purpose of calling variants from whole exome sequencing data. We will go step-by-step through the best practices guide from the Genome Analysis Toolkit. After completing this tutorial, you should feel comfortable calling variants from data generated in your own labs.

Pre-requisites: "Introduction to Unix" and "Introduction to Scientific Computing for Genomics" (or equivalent experience).

Single Cell and PCR Based Technologies
Instructors: Dr. Holly Dressman, Co-Director, Sequencing and Genomic Technologies
Cost:  FREE

Single cell expression profiling can be facilitated through the automation of the Fluidigm C1 System.  The system allows one to capture single cells and explore gene expression profiling through the use of qPCR or RNA sequencing analysis.   The technology will be discussed as well as how it can be applied in your research.  Pre-requisites: Basic understanding of molecular biology. PCR, quantitative PCR and droplet-digital PCR technologies will be discussed along with examples on which technology would best fit your research.

Bioinformatics Tools for Proteomics & Metabolomics
Instructors: Dr. Erik Soderblom and Dr. Will Thompson, Duke Proteomics and Metabolomics 
Cost: Free

Liquid chromatography coupled with mass spectrometry (LC/MS) is a versatile tool for the qualitative and quantitative characterization of peptides, proteins and metabolites for both basic and clinical research projects. One of the most important considerations in being able to translate LC-MS datasets into meaningful biological observations is to effectively use open source software packages and/or online resources geared toward LC-MS based datasets.  This GCB Academy session is designed as a complement to GCB Academy course Fundamentals of Mass Spectrometry for Proteomic and Metabolomic Analyses (Nov 7th) and GCB Academy course “Experimental Design: Get the most out of your proteome” (Nov 8th) and is intended for users of the Proteomics and Metabolomics Shared Resource who have or plan on generating LC/MS based Proteomic or Metabolomic Datasets with the Shared Resource. This first portion of the course will focus on the effective use of Scaffold to characterize qualitative proteomic datasets. This will include an overview of Scaffold and features such as interpretation of spectral matches at a protein or peptide level, gene ontology classification, homology matching, spectral count data, and data export. The second portion of the course will cover common proteomic and metabolomic data analysis strategies from supplemental data (typically .xlsx file formats from Rosetta Elucidator) provided as part of the Shared Resource’s quantitative proteomic workflows. This will include an overview of the typical features of a quantitative data return document, various data summarization levels, calculating peptide/protein relative fold-changes and p-values, exporting data for motif analysis (PTM specific datasets), and performing Principle Component Analysis (PCA) and 2D Clustering within JMP Pro.

Practical Postmortem Analysis Exercises in Critical Examination of Proteomics Data
Instructors: Dr. Erik Soderblom and Dr. Will Thompson, Duke Proteomics and Metabolomics 
Cost: Free

Critical review of a Proteomics data analysis presents unique challenges because of the complex workflows involved in going from raw mass spectrometry data to results interpretation.  Using tools discussed in the “Bioinformatics Tools” course, this class will work to ‘deconstruct’ a proteomics experiment which has had flaws in the analysis and interpretation.  By finding the errors in data analysis and interpretation, the goal of this case study will be to become more aware of many common pitfalls in proteomics data analysis, and enhance your skills in reviewing proteomics datasets which are becoming much more common in the peer-reviewed literature.  The material will be guided, but hands-on participation is expected.  Laptops required.  Prerequisites: Attendance at “Fundamentals” and “Experimental Design” classes recommended but not required, attendance at “Bioinformatics Tools” highly recommended.