Single Cell Gene Expression


Fluidigm Services

Fluidigm Technology

The world's first automated solution for single-cell genomics research, the C1 Single-Cell Auto Prep system employs innovative microfluidic technology to rapidly and reliably isolate, process and prepare individual cells for genome and transcriptome analysis. With just one instrument, you can isolate cells, stain them and prepare template for sequencing or qPCR. Please refer to their website to learn more about Fluidigm C1 assays.

Fluidigm’s Integrated Fluidic circuit (IFC) and NanoFlex Valve technology enables the rapid, efficient, highly parallel, and reproducible analysis of tens-to-hundreds of genetic markers, across hundreds-or-thousands of DNA samples, in hours instead of days or weeks. Fluidigm’s technology supports genomics-based applications such as high throughput RT-PCR, single-cell gene expression and RNA-seq library preparation, SNP genotyping. Other available technologies include copy number variation, protein expression and digital PCR.

Experiment Design

Please contact us at microarraylab@duke.edu to discuss your experimental design and service needs prior to submitting samples to the facility. We will work with you to map out the subsequent steps of your project, and can coordinate with other GCB core facilities as needed for DNA sequencing, statistical analysis, and data storage. Submissions will only be accepted after a meeting has occurred.

Sample Preparation

Please reference the Single Cell Preparation Guide for details on sample preparation.

Service Pricing and Summary

Primers