Beta Globin Reduction
The Ambion GLOBINclear™ Whole Blood Globin Reduction Kits, human remove the globin transcripts which represent up to 70% of the mRNA in whole-blood. The kit will rapidly depletes >95% of the alpha- and beta-globin mRNA from whole-blood total RNA. The resulting RNA is superior for synthesizing labeled cDNA/cRNA for array analysis and quantitative RT-PCR. Up to 50% more genes are detectable on Affymetrix® GeneChip® Arrays, and it requires no RNase H treatments, which can degrade mRNA and alter expression profiles, or expensive peptide nucleic acid (PNA) oligomers.
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Sample Requirements, Tubes & Protocols
- Samples: total RNA >3 ug (minimal: >1ug)
- Instruction Manual: GLOBINclear Kit
Online Submission Form
Investigators that wish to use Beta Globin Reduction service should use our online submission forms to initiate the process.