Agilent Microarray Sample Submission Requirements

Agilent Microarray Sample Submission Requirements

Gene Expression Services
  • For total RNA, the concentration and 260/230 absorbance ratios will be checked on the Nanodrop.  The Agilent Bioananlyzer will be used to check the RNA integrity.  Genomic DNA will be analyzed for concentration on the Qubit 2.0 Fluorometer.
  • All samples must be resuspended in a volume of 5-8 ul of nuclease-free water.  Please provide an extra 3 ul aliquot of each sample in a separate 1.5 ml tube for quality assessment.  If sample is not in water, please specify on submission form.
Sample TypeProcessing TypeStarting AmountStarting ConcentrationStarting VolumeExtra QC Sample Required
Total RNALIQA 1-color100-200 ng>50 ng/ul1.5 ulYes, 3 ul
Total RNALIQA 2-color100-200 ng>50 ng/ul1.5 ulYes, 3 ul
Total RNAmiRNA100-200 ng>50 ng/ul2 ulYes, 3 ul
Genomic DNACGH*500 ng>50 ng/ul26 ulYes, 3 ul

 

*DNA gel picture must be submitted with genomic DNA

Hybridization Only Services

For submission of samples that will be hybridized and arrayed, please submit Cy3 and Cy5 combined labeled gDNA.

Processing TypeStarting AmountStarting ConcentrationStarting VolumeExtra QC Sample Required
CGH for 2x105k arrayFollow Appropriate ProtocolFollow Appropriate Protocol79 ulNo
CGH for 4x44k arrayFollow Appropriate ProtocolFollow Appropriate Protocol39 ulNo

 

Please contact us at microarraylab@duke.edu if you have any questions.