Agilent Microarray Sample Submission Requirements
Gene Expression Services
- For total RNA, the concentration and 260/230 absorbance ratios will be checked on the Nanodrop. The Agilent Bioananlyzer will be used to check the RNA integrity. Genomic DNA will be analyzed for concentration on the Qubit 2.0 Fluorometer.
- All samples must be resuspended in a volume of 5-8 ul of nuclease-free water. Please provide an extra 3 ul aliquot of each sample in a separate 1.5 ml tube for quality assessment. If sample is not in water, please specify on submission form.
|Sample Type||Processing Type||Starting Amount||Starting Concentration||Starting Volume||Extra QC Sample Required|
|Total RNA||LIQA 1-color||100-200 ng||>50 ng/ul||1.5 ul||Yes, 3 ul|
|Total RNA||LIQA 2-color||100-200 ng||>50 ng/ul||1.5 ul||Yes, 3 ul|
|Total RNA||miRNA||100-200 ng||>50 ng/ul||2 ul||Yes, 3 ul|
|Genomic DNA||CGH*||500 ng||>50 ng/ul||26 ul||Yes, 3 ul|
*DNA gel picture must be submitted with genomic DNA
Hybridization Only Services
For submission of samples that will be hybridized and arrayed, please submit Cy3 and Cy5 combined labeled gDNA.
|Processing Type||Starting Amount||Starting Concentration||Starting Volume||Extra QC Sample Required|
|CGH for 2x105k array||Follow Appropriate Protocol||Follow Appropriate Protocol||79 ul||No|
|CGH for 4x44k array||Follow Appropriate Protocol||Follow Appropriate Protocol||39 ul||No|
Please contact us at email@example.com if you have any questions.