Sequencing and Genomic Technologies

Sequencing and Genomic Technologies

scientist in the lab

Next-Generation Sequencing Solutions
Illumina, PacBio, NGS Library preparation including single-cell RNA-seq

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The Sequencing and Genomic Technologies Shared Resource is a basic research oriented core facility affiliated with the Duke Cancer Institute (DCI). This Shared Resource has a track record more than a decade-long of providing constantly updated, state-of-the-art genomic services. The Sequencing and Genomic Technologies Shared Resource offers the full range of genomic technologies, making it much simpler for researchers to find the right service for their needs.

Director Nicolas Devos is available for face-to-face project consultations on campus in the CIEMAS building or at our new facility. 

Schedule a project consulation

Service update

Due to the low demand for sequencing on our Illumina HiSeq 2500, it has become difficult to keep this instrument operational.  As a consequence, our HiSeq 2500 will, unfortunately, be retired.

We apologize for the inconvenience this may cause.  The Sequencing and Genomic Technologies Shared Resource team is here to answer any questions you may have, and more importantly, help you transition your sequencing to different Illumina instruments. Our Shared Resource owns other Illumina instruments that can be used in place of the HiSeq 2500, such as the NextSeq. If you have concerns that your libraries won’t sequence well on other instruments, please let us know.

Nucleic Acid Extraction Services

We now provide Nucleic Acid extraction services on our QIAsymphony robot. The new extraction services provide researchers with fast turnaround time, high reproducibility through standardized processing of large sample batches (96 samples at a time) and seamless integration with our NGS library preparation services.

We currently offer total RNA extraction from blood samples in PAXgene tubes, total RNA extractions from cell pellets and miRNA extraction from serum/plasma.  Please visit our sample requirement page for more information on how to submit your samples.

Don’t hesitate to contact us if you have large amount of samples to extract.  The QIAsymphony can automate many types of DNA and RNA extractions from a broad range of sample types.

Average Time in Queue

Client-Submitted LibrariesCore-Created Libraries
Mi-Seq1 week3-4 weeks
NextSeq1 week3-4 weeks
HiSeq 2500 Rapid Run1 week3-4 weeks
HiSeq 4000 full flow cell1 week3-4 weeks
HiSeq 4000 by the lane3-4 weeks3-4 weeks
NovaSeq 6000 full flow cells1 week3-4 weeks
Novaseq 6000 by the laneDependent on how quickly a flow cell can be filledDependent on how quickly a flow cell can be filled
PacBioSequelNot applicable2 weeks

Announcements

November 16, 2018

The Sequencing and Genomic Technologies Shared Resource will be closed December 24, 2018 - January 1, 2019. During this time, we will not be receiving or processing samples.

February 28, 2018
The Sequencing Core has acquired a brand new QIAsymphony to perform high throughput nucleic acid extractions. We currrently have a service for RNA extractions from blood collected in PAXgene tubes. The QIAsymphony can automate many types of DNA and RNA extractions from a broad range of sample types. Contact us to discuss potential other nucleic acid extraction services.