Next-Generation Sequencing Solutions
Illumina, PacBio, NGS Library preparation including single-cell RNA-seq
The Sequencing and Genomic Technologies Shared Resource is a basic research oriented core facility affiliated with the Duke Cancer Institute (DCI). This Shared Resource has a track record more than a decade-long of providing constantly updated, state-of-the-art genomic services. The Sequencing and Genomic Technologies Shared Resource offers the full range of genomic technologies, making it much simpler for researchers to find the right service for their needs.
Director Nicolas Devos is available for face-to-face project consultations on campus in the CIEMAS building or at our new facility.
Due to the low demand for sequencing on our Illumina HiSeq 2500, it has become difficult to keep this instrument operational. As a consequence, our HiSeq 2500 will, unfortunately, be retired.
We apologize for the inconvenience this may cause. The Sequencing and Genomic Technologies Shared Resource team is here to answer any questions you may have, and more importantly, help you transition your sequencing to different Illumina instruments. Our Shared Resource owns other Illumina instruments that can be used in place of the HiSeq 2500, such as the NextSeq. If you have concerns that your libraries won’t sequence well on other instruments, please let us know.
Nucleic Acid Extraction Services
We now provide Nucleic Acid extraction services on our QIAsymphony robot. The new extraction services provide researchers with fast turnaround time, high reproducibility through standardized processing of large sample batches (96 samples at a time) and seamless integration with our NGS library preparation services.
We currently offer total RNA extraction from blood samples in PAXgene tubes, total RNA extractions from cell pellets and miRNA extraction from serum/plasma. Please visit our sample requirement page for more information on how to submit your samples.
Don’t hesitate to contact us if you have large amount of samples to extract. The QIAsymphony can automate many types of DNA and RNA extractions from a broad range of sample types.
Average Time in Queue
|Client-Submitted Libraries||Core-Created Libraries|
|Mi-Seq||1 week||3-4 weeks|
|NextSeq||1 week||3-4 weeks|
|HiSeq 2500 Rapid Run||1 week||3-4 weeks|
|HiSeq 4000 full flow cell||1 week||3-4 weeks|
|HiSeq 4000 by the lane||3-4 weeks||3-4 weeks|
|NovaSeq 6000 full flow cells||1 week||3-4 weeks|
|Novaseq 6000 by the lane||Dependent on how quickly a flow cell can be filled||Dependent on how quickly a flow cell can be filled|
|PacBioSequel||Not applicable||2 weeks|