Validation of Expressed Variants by Targeted Proteomics

The expression of non-synonymous variants can be difficult or impossible to validate using exisiting immunoassays. Targeted proteomics can be used to specifically and precisely measure single amino acid changes. Assays are adaptable to most biological matrices, highly multiplexible and require small protein quantities.

  • Full support - experimental design, sample preparation and analysis, initial data analysis
  • State-of-the-art LC/MS/MS based technology using Multiple Reaction Monitoring
  • Assays use internal standards for each peptide of interest
  • High throughput, high sensitivity, high reproducibility and rigorous quality control
  • Can be multiplexed for hundreds of peptides

Targeted Quantitation of Expressed Variants of Pulmonary Surfactant Protein A

Quantitation of K223 variant of SP-A with internal standard

Line graph showing quantitation of K223 variant of SP-A with internal Standard

Identification of individuals with homozygous or heterozygous expression of SP-A variants

Chart with identfication of individuals

Workflow

  1. Sample Prep
  2. LC/MS/MS
  3. Data Workup

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