Unbiased ('omic) Differential Expression Proteomics in Biofluids

Quantitative proteomics of biofluids can be an important discovery tool for characterizing disease and response to therapy and identifying biomarker candidates. Methods are amendable to animal models and human samples. Typically, several hundred to approximately 1,000 proteins can be quantified in biofluids after immunodepletion of abundant proteins (e.g. albumin, IgG, transferrin).

  • Full support - experimental design, sample preparation and analysis, initial data analysis
  • State-of-the-art LC/MS/MS based technology using rigorous quality control metrics
  • Immunodepletion of abundant proteins increases coverage of biofluid proteomes
  • Expertise with plasma, CSF, nasal and bronchoalveolar lavage, synovial fluid

Quantitation of Bronchoalveolar Lavage in Idiopathic Pulmonary Fibrosis

Immunodepletion of abundant plasma proteins

Immunodepletion of abundant plasma proteins

Cluster analysis distinguishes IPF and normal control groups

Cluster analysis distinguishes IPF and normal control groups

Workflow

  1. Sample Prep with immunodepletion
  2. LC/MS/MS
  3. Data Workup

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