Targeted Metabolomics Oxylipins/Eicosanoids/Bioactive Lipids

Targeted differential expression metabolomics characterizes the changes in the metabolome as a function of biological challenges. These experiments can be hypothesis generating or hypothesis testing. Eicosanoids have a profound biological effects, including inflammation and immune system response. There are multiple families of oxylipins, including prostaglandins, thrombaxanes and leukotrienes.

  • Full support - experimental design, sample preparation and analysis, initial data analysis
  • State-of-the-art LC/MS/MS based technology using multiple reaction monitoring
  • Rigorous quality control
  • High throughput, high sensitivity and high reproducibility

Internal Standards

  • d4 6-keto PGF1a
  • d4-TXB2
  • d4-PGF2a
  • d4-PGE2
  • d4-PGD2
  • d4-LTB4
  • d4-(±)12,13-DiHOME
  • d4-(±)9,10-DiHOME
  • d22-14, 15-DiHETrE
  • d6-20-HETE
  • d4-9(S)-HODE
  • d8-12(S)-HETE
  • d8-5(S)-HETE
  • d7-5-KETE
  • d4 15-deoxy-12,14-PGJ2

Analyte Name

  • 6-keto PGF1a
  • PGD2
  • PGE2
  • TXB2
  • 11ß-PGF2a
  • 13,14-dihydro-15-keto PGD2
  • 13,14-dihydro-15-keto PGE2
  • 13,14-dihydro-15-keto PGF2a
  • Tetranor-PGFM
  • 12,13-DiHOME
  • 5(S),6(S)-Lipoxin A4
  • (+/-) 5-iPF2a-VI
  • LTD4
  • LTE4
  • 11-HETE
  • 12-HETE
  • 15-HETE
  • 5-HETE
  • 8-HETE
  • 9-HETE
  • 13-HODE
  • 9-HODE
  • 12(S)-HpETE
  • 13-HpODE
  • 15-HpETE
  • 5(S)-HpETE
  • 9-HpODE
  • 14,15-DiHETrE
  • 12(13)-EpOME
  • 10(S), 17(S)-DiHDoHE
  • NeuroprotectinD1
  • ResolvinD1
  • ResolvinE1
  • Maresin 1

Workflow

  1. Oxylipin Assay
  2. LC/MS/MS

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