Pricing For Core Service Support for Duke and Non-Duke Scientists

Note: Scientific collaborations with academic, government, and non-profit laboratories outside Duke are welcome. Please contact Director Dr. Arthur Moseley for details.

Please note: Prices below are based on an a-la-carte menu of services to maximize flexibility in experimental designs and comply with auditing regulations. Most analyses will require combinations of these services. Please see the FAQs for additional information. All prices are effective July 1, 2018.

Sample Preparation (Proteomics)

Mini-Bradford Assay (per plate, 20 samples max)$43$69
   - NOTE - Bradford Assays required for all solution phase analyses
General Sample Preparation - Proteomics (cost per method)$53$85
           In-Solution Digestion*
            FFPE Protein Extraction
           In-situ/bead-bound digestion
Tissue Disruption/Lysis and Protein Extraction (RapiGest, Urea,TriZol, or Bead Blasting)       $26$42
In-Gel Digestion (per spot or band)*$59$94
S-trap Digestion$95$152
Sample Cleanup Procedures (cost per method)$48$77
            Solid Phase Extraction (C18, HLB, MCX)
            Buffer exchange by ZebaSpin
            Protein Precipitation and Resuspension
            Biotin-Avidin enrichment
Amicon MW Cutoff Filtration$60$96
TiO2 enrichment for phosphopeptides$57$91
Glycopeptide enrichment$97$155
1D PAGE Gel Separation (Protein Level)$115$184
OFFGEL pl Fractionation (12 or 24 fraction, peptide or protein)ContactContact
GELFREE Molecular Weight Fractionation (protein level)ContactContact
Offline Fractionation (C18 or SCX)$178$285
Biofluid Immunodepletion, including MW filter cleanup
            Spin Column Format (Pierce Hu-12 or Agilent MU-3)$126$202
            LC Format (Agilent MARS-14)$194$310
Antibody-Based Enrichment (not including antibody reagent costs)$71$114
*Additional reagents charge for digestions with enzymes other than Trypsin*ContactContact

Sample Preparation (Metabolomics)

MeOH Precipitation with Internal Standards - Biofluidsincludedincluded
Solid Sample Solubilization (Cells, Tumor) by Bead Blasting, Probe Sonication, or Tissue Tearing$26$42
Mini-Bradford Assay (per plate, 20 samples max)$43$69
  -NOTE - this is used to normalize to total biomass
Specialized Per-Sample Preparation for Specific Assays is Included in the Cost/Samplen/an/a
Pharmacokinetics Plate Prep (biofluids)$68$109

Qualitative Analyses (Proteomics)

LC-MS/MS analysis (simple in-gel digest - very fast gradient)$84$134
LC-MS/MS analysis (in-gel or solution digest of simple sample - fast gradient)$146$234
LC-MS/MS analysis (moderately complex - medium gradient)$185$296
LC-MS/MS analysis (complex sample - slow gradient)$218$349
LC-MS/MS analysis (very complex sample - very slow gradient)$264$422
2D LC-MS/MS (5-fraction)$645$1,032
2D LC-MS/MS (10-fraction)$1,063$1,701
       On-line LC/LC Setup Fee (per order)$1,105$1,768
Molecular Weight Determination (per sample)$49$78
         MW Setup Fee (per order)$130$208
ZipChip Profiling Method$67$107

Quantitative Analyses, Unbiased (Proteomics)

LC-MS/MS Differential Expression (per sample), 15-min. gradient$98$157
LC-MS/MS Differential Expression (per sample), 30-min. gradient$237$379
LC-MS/MS Differential Expression (per sample), 60-min. gradient$276$442
*LC-MS/MS Differential Expression (per sample), 90-min. gradient$315$504
LC-MS/MS Differential Expression (per sample), 120-min. gradient$354$566
2D LC-MS/MS Differential Expression (3-fraction)$654$1,046
2D LC-MS/MS Differential Expression (5-fraction)$918$1,469
2D LC-MS/MS Differential Expression (10-fraction)$1,321$2,114
         On-line LC/LC Setup Fee (per order)$1,105$1,768
Quantitative 1D Workup - Elucidator (analysis per sample)$135$216
Quantitative 2D Workup - Elucidator (analysis per sample)$200$320

Quantitative Analyses, Targeted (Proteomics)

*Stable Isotope Labeled Peptides are provided at third party vendor cost. Methods are developed and performed on nanoAcquity UPLC and TQ-S triple quadrupole mass spectrometer unless otherwise stated. The shortest method possible is used for quantification of the number of analytes requested, using retention-time scheduled MRM technology. Typically, at least two peptides per protein and three transitions per peptide are used for MRM quantitative assays.

Targeted (MRM) Quantitation Method Development, priced per hourContactContact
MRM Quantitative Data Collection (30 min gradient)$114$182
MRM Quantitative Data Collection (60 min gradient)$153$245
MRM Quantitative Data Collection (90 min gradient)$186$298
MRM Data Analysis in Skyline, priced per hourContactContact

Quantitative Analysis (Metabolomics)

All prices are on a per-sample basis.

AbsoluteIDQ p180 Panel (kit from Biocrates, Inc.)*$53$85
Absolute IDQ p400 HR Panel (kit from Biocrates, Inc.)*$72$115
Bile Acids Panel (kit from Biocrates, Inc.)*$41$66
Oxylipins/Eicosanoid Panel**$144$230
Energy Metabolite Panel$63$101
Methionine Pathway Panel**$91$146
Hydroxycholesterols Panel**$130$208
27-Hydroxycholesterol quantitation**$79$126
Fatty Acids Panel$78$125
Fast HR Metabolite Profiling (RPLC OR HILIC, ESI+ OR ESI-)$144$230
High Resolution Polar Metabolite Profiling, ESI+$203$325
High Resolution Lipid Profiling, ESI+ and ESI-$249$398
PK Analysis - method developmentContactContact
     per sample with in-house developed method**$28$45
Polyamine Panel**$62$99
Short Chain Fatty Acids Analysis**$61$98
Sphingosine S1P Panel**$45$72
ZipChip Profiling Method$67$107

*These assays require additional purchase of kit consumables from Biocrates, Inc. Negotiated prices may vary significantly depending on the number of samples to be analyzed.

**These assays will incur an extra charge for running a standard curve, quality control injections, and blanks.

Data Analysis

Custom Database Setup for MASCOT or Identify$26$42
Additional Database Searches$32$51
Consulting and Extensive Data Mining (per hour)$71$114

FAQ Total Pricing Examples

Total Pricing for Common Workflows:

Digestion and Very Fast LC-MS/MS of 1D Gel Band for simple ID$143$229
     (In-gel Digestion, 20-min gradient LC-MS/MS)
Analysis of Immunoprecipitation Samples (2) by 90-min gradient$585$936
     (Bradford assay, in-solution digestion, 90-min LC-MS/MS)
Quantitative 1DLC Analysis between two conditions, requiring n=3 biol samples per condition. 1DLC, with coverage of ~1,000 proteins$3,061$4,898
      (Bradford assay, in-solution digestion, quantitative LC-MS/MS, and quantitative workup including statistics and M&M for publication)
Qualitative Phosphorylation Mapping from a Simple Sample, i.e. gel band$301$482
       (In-gel digestion, TiO2 enrichment, Medium 1D LC-MS/MS)
Qualitative Phosphorylation Mapping from a Complex Sample$371$594
       (Bradford, macroscale digestion, TiO2 enrichment, Slow 1D LC-MS/MS)

For additional pricing examples for your particular study, please consult with the Proteomics and Metabolomics Shared Resource.