Tutorial Presentations
Genome Academy 2013
Lecture 1 -
Lecture 2 -
Lecture 3 -
Differential Expression ProteomicsFundamentals of Mass Spectrometry
A general introduction to mass spectrometry, including the different types of ionization sources and mass analyzers typically used in state-of-the-art biological, pharmaceutical, and environmental mass spectrometry. This presentation is courtesy of Dr. Doug Sheeley at NIH. 37 slides. View
Fundamentals of Protein Chemistry
An introduction to protein chemistry, including amino acid and peptide chemistry, transcription and translation, post-translational modifications and classical analytical methods. Presentation courtesy of Dr. Doug Sheeley at NIH. 28 slides. View
Capillary Liquid Chromatography - Mass Spectrometry
An in-depth presentation on the practice of performing capillary liquid chromatography coupled to mass spectrometry specifically for application to biological mass spectrometry and proteomics. Includes a general background about the sensitivity and performance characteristics of state of the art LC-MS systems, as well as hints about best practices for applied LC-MS using commercial instrumentation. This presentation is courtesy of Dr. Arthur Moseley at Duke University. 62 slides. View
Fundamentals of Proteomics
Presentation describes the emerging proteomics technologies in use in proteomics laboratories across the globe for interaction proteomics and expression proteomics. Topics include using 2D gels coupled to LC-MS and MALDI-MS, gel-free proteomics (LC-MS/MS and LC/LC-MS/MS) and MALDI tissue "imaging", with many references to jounral articles for more in-depth information. This presentation is courtesy of Dr. Arthur Moseley at Duke University. 34 slides. View
Protein Identification by Database Searching
An in-depth description of how "bottom-up" proteomics uses protein databases along with peptide fragmentation data from MS/MS experiments to determine sequence information for a protein or protein mixture. This presentation is courtesy of Dr. John Cottrell at Matrix Sciences, Ltd. 31 slides. View
Quantitative Proteomics
Presentation describes the three most popular techniques for rlative and/or absolute protein quantitation using mass spectrometry, including isotope labeling strategies (iCAT, iTRAQ, SILAC, etc.), label-free (direct) quantitation, and targeted quantitation using multiple reaction monitoring (MRM). This presentation is courtesy of Dr. Arthur Moseley at Duke University. 41 slides. View
Bioinformatic Tools for Proteomics
This presentation describes several of the basic bioinformatic tools used in proteome analysis, with a focus on "open-source" tools. This presentation is courtesy of Dr. Arthur Moseley at Duke University. 43 slides. View