PowerPoint Presentations

Tutorial Presentations

Genome Academy 2013

Lecture 1 - 

Lecture 2 - 

Lecture 3 - 

Fundamentals of Mass Spectrometry

A general introduction to mass spectrometry, including the different types of ionization sources and mass analyzers typically used in state-of-the-art biological, pharmaceutical, and environmental mass spectrometry. This presentation is courtesy of Dr. Doug Sheeley at NIH. 37 slides. View

Fundamentals of Protein Chemistry

An introduction to protein chemistry, including amino acid and peptide chemistry, transcription and translation, post-translational modifications and classical analytical methods. Presentation courtesy of Dr. Doug Sheeley at NIH. 28 slides. View

Capillary Liquid Chromatography - Mass Spectrometry

An in-depth presentation on the practice of performing capillary liquid chromatography coupled to mass spectrometry specifically for application to biological mass spectrometry and proteomics. Includes a general background about the sensitivity and performance characteristics of state of the art LC-MS systems, as well as hints about best practices for applied LC-MS using commercial instrumentation. This presentation is courtesy of Dr. Arthur Moseley at Duke University. 62 slides. View

Fundamentals of Proteomics

Presentation describes the emerging proteomics technologies in use in proteomics laboratories across the globe for interaction proteomics and expression proteomics. Topics include using 2D gels coupled to LC-MS and MALDI-MS, gel-free proteomics (LC-MS/MS and LC/LC-MS/MS) and MALDI tissue "imaging", with many references to jounral articles for more in-depth information. This presentation is courtesy of Dr. Arthur Moseley at Duke University. 34 slides. View

Protein Identification by Database Searching

An in-depth description of how "bottom-up" proteomics uses protein databases along with peptide fragmentation data from MS/MS experiments to determine sequence information for a protein or protein mixture. This presentation is courtesy of Dr. John Cottrell at Matrix Sciences, Ltd. 31 slides. View

Quantitative Proteomics

Presentation describes the three most popular techniques for rlative and/or absolute protein quantitation using mass spectrometry, including isotope labeling strategies (iCAT, iTRAQ, SILAC, etc.), label-free (direct) quantitation, and targeted quantitation using multiple reaction monitoring (MRM). This presentation is courtesy of Dr. Arthur Moseley at Duke University. 41 slides. View

Bioinformatic Tools for Proteomics

This presentation describes several of the basic bioinformatic tools used in proteome analysis, with a focus on "open-source" tools. This presentation is courtesy of Dr. Arthur Moseley at Duke University. 43 slides. View