Illumina GA (Solexa)
The IGSP Sequencing Facility is pleased to offer services on our two Illumina Genome Analyzer II's (aka Solexa) since September of 2008.
Illumina Sequencing, a short-read platform, is excellent for producing 50 million reads at the current read length of 36 bp in one 2-day instrument run.
Short-read sequencing is great for medical re-sequencing, digital gene expression, small RNA, tag profiling, ChIP Seq., and methylation studies. For an overview please see Illumina Sequencing.
Steps to Running your Illumina Sequencing Project at the Duke IGSP Sequencing Facility:
1. Decide what type of project you would like to do. The link above has some applications advice.
2. Review your bio-informatics capabilities or contacts. Management of Illumina seqeuncing data is labor-intensive. The lab is expecting to hire a bio-informatics consultant in the near future. Tonya Severson, a Facility technician, has compiled a list of Next-Gen Bio-Info Resources to give you an idea of what third-party software is available so far.
3. Have a look at our current Illumina Sequencing, IGSP Prices .
4. Contact Lisa Bukovnik to discuss your project.
5. Prepare your sample according to your specific Illumina library preparation protocol. Kits can be ordered directly from Illumina, contact Steve Matta for a quote. Unfortunately, at this time our lab is not staffed to offer library preparation service for Illumina libraries.
6. Submit your sample to the Facility to get in the queue. If you are mailing a sample to us, send it well-padded and on dry ice. Each sample is handled with custom care, so turn-around time varies.
7. When you deliver your samples you can request which sequence format(s) you need (fasta, fastq, or scarf) and whether you need ascii or numeric quality format(s). Your data are returned to you as tar.gz files.
8. The customer should back up data upon receipt and verify the data content and format are sufficient. After 10 business days your data will be deleted from our server.