Screening Services
Our facility operates on an investigator-assisted screening model. The researcher works in the Facility during the process and is intimately involved in all aspects of the screen. We assist in all steps of the screening process including assay development, pre-screen validation, screening, and data analysis.
- High-Throughput Screening (HTS) assay development: RNAi based high-throughput screening (HTS) is a powerful tool for functional genomics, however it is a process involving many steps, most notably assay development. The development of each HTS assay system takes time, and can range from several months to a year. The importance of a robust HTS assay cannot be overstated; therefore the process must be started with the knowledge that it involves a significant time investment. We have extensive experience in assay development and look forward to working with you to adapt your assay system to HTS. This service is included in the price of a whole-genome or sub-genome siRNA screen, but can also be utilized for developing a HTS assay for chemical or small molecule screening.
- Whole-genome siRNA screening: We currently offer screening of the human genome utilizing the Qiagen Human whole-genome siRNA library. The library targets approximately ~22,000 genes using 384-well format. Cost is dependant on the complexity of the HTS assay system as well as the number of “hits” moved into the secondary validation screen. Cost estimation is complex and determined on a case-by-case basis. Pricing will typically range from $12,000-$20,000.
- Sub-genome siRNA screening: We also offer a sub-genomic screen targeting ~1400 kinases, phoshpatases, and GPCRs.