Duke Proteomics Core Facility

Instrumentation

Instrumentation

Ultrahigh Pressure Nanoscale Capillary

LC Ultra-performance Nanoscale Liquid Chromatography - nanoAcuity UPLC

- Four NanoAcquity systems and two Multidimensional NanoAcquity systems are available in our lab

 

nanoAcquity
The nanoACQUITY UPLC System

(nanoAcquity Material from Waters Corporation Website)

The nanoACQUITY UltraPerformance LC® (UPLC®) System is designed for nano-scale, capillary, and narrow-bore separations to attain the highest chromatographic resolution, sensitivity, and reproducibility.

Direct nano-flow offers significant improvements over conventional nano-flow separations technologies. You’ll see improved peak capacity and peak shape, and increase the number of components that can be detected per separation.

The system’s 10,000 psi operating pressure capability allows for superior high-peak capacity separations by operating longer columns packed with sub-2 micron particles. It is optimized for high-resolution identification and 2D-LC separations at precise nano-flow rates:

 

Nano-flow Rates From: On nanoACQUITY UPLC Columns*:
200 nL/min to 100 µL/min without flow splitting
  • 75 µm to 1 mm internal diameter
  • 100 mm to 250 mm long
*Available with BEH, Atlantis, and Symmetry chemistries

 

The nanoACQUITY UPLC System provides solutions for biomarker discovery and proteomics applications, for protein identification and characterization.

nanoACQUITY UPLC System Features:

  • Customize your throughput and condition requirements with comprehensive system components including the nanoACQUITY® Heating and Trapping Module, the Sample Manager, the Binary Solvent Manager, and the Auxiliary Solvent Manager (optional).
  • Monitor system performance with enhanced on-board diagnostics.
  • Extend high-sensitivity nano-scale MS analyses: from the Quattro Premier™ XE tandem quadrupole mass spectrometer for routine quantification; to the SYNAPT™ HDMS™ System for cutting edge applications; to the IdentityE High Definition Proteomics System for protein identification.
  • Predictive system support with Connections INSIGHT® remote intelligent services.
  • Instrument control and data processing with MassLynx™ Software.
  • Easily compatible with third-party MS solutions.
  • FlexCart lets you move nanoACQUITY UPLC system from one MS to another.
  • Stand-alone nanoACQUITY UPLC console provides easy instrument control.