DNA Microarray Core Facility

RNA Quality Check

RNA Quality Check

Our RNA quality check service includes:

  • Quality check with NanoDrop to assess sample concentration and purity.
  • Quality check with Agilent Bioanalyzer to assess sample integrity.
  • Quality check report to summarize sample quality. 

Contact microarraylab@duke.edu or call (919)668-1583 for pricing.

Preferred RNA isolation methods for microarray analysis

NanoDrop Spectrophotometer (ND-8000)

NanoDrop 8000

 

NanoDrop is a cuvette free spectrophotometer and fluorospectrometer system used to quantitate micro-volumes of sample. NanoDrop 8000 allows up to eight micro-volume samples (0.5 ul - 2.0 ul) to be measured simultaneously.

 

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Agilent Bioanalyzer

Agilent RNA microfluidic chip

The Agilent Bioanalyzer RNA assay leverages microfluidics technology, enabling the quality analysis of RNA using only 1ul of sample. The assay is run on the Agilent 2100 Bioanalyzer instrument and utilises the Agilent 2100 Expert Software to analyze and display results. An RNA Integrity Number (RIN score) is generated for each sample on a scale of 1-10 (1=lowest; 10=highest) as an indication of RNA quality. The 18s/28s ratio and an estimation of concentration is also produced.

Chip Type RNA type Concentration Range max # of samples per chip
RNA Nano Chip totalRNA 25-1000ng/ul 12
RNA Pico Chip totalRNA 0.05-5ng/ul 11
smallRNA Chip totalRNA 10-100ng/ul 11

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