Real-Time PCR (RT-PCR) (reverse transcription-polymerase chain reaction) is the most sensitive technique for mRNA detection and quantitation currently available. Compared to Northern blot analysis and RNase protection assay, RT-PCR can be used to quantify mRNA levels from much smaller samples and, ultimately, quantitation of RNA from a single cell.
The benefit of RT-PCR is the ability to obtain quantitative results from the sensitive PCR assay. Since Real-Time PCR quantitates reaction products for each sample in every PCR cycle, the result is an amazingly broad dynamic range, with no user intervention required. Data analysis, including standard curve generation and copy number calculation, is performed automatically by the SDS software connected to our ABI 7900 HT RT-PCR machine.
Use of the ABI 7900 HT is available for Real-Time assays or plate reads. This is a charged instrument service, please refer to pricing. The RT-PCR machine is located in 2196AB CIEMAS Building. First time users please email Heather Hemric to schedule a tutorial before using the machine. Online Signup is required to schedule use of this instrument.
Full RT-PCR service with plates prepared with liquid handler is available. Please check our Liquid Handling Service page for details.
Contact firstname.lastname@example.org or call (919)668-1583 for pricing
SDS 2.3 is available to RT-PCR customers free of charge